Injongo: Ukufunda umphumo wecandelo lokunciphisa i-tumor I (AAVC-I) kwi-proliferation inhibition kunye ne-apoptosis yomhlaza wemiphunga yabantu iiseli ze-A549.Iindlela: Indlela ye-MTT yayisetyenziselwa ukufumanisa izinga le-24h kunye ne-48h yokuvimbela iiseli ze-A549 nge-AAVC-I kwiindawo ezahlukeneyo.
I-HE staining kunye ne-Hoechst33258 i-fluorescent staining yasetyenziselwa ukujonga i-apoptosis evela kwi-morphology;I-Immunohistochemistry yayisetyenziselwa ukufumanisa utshintsho kwi-BAX protein expression.Iziphumo: I-MTT ibonise ukuba i-AAVC-I inokuthintela ukwanda kweeseli ze-A549 ngexesha kunye nokuxhomekeka kwedosi.Emva kokunyangwa kwe-AAVCI ye-24h, i-nucleus contraction, i-nyukliya hyperstaining kunye nemizimba ye-apoptotic yayibonakala phantsi kwe-microscope.I-Immunohistochemistry ibonise ukuba i-avareji ye-optical density value inyuke ngokunyuka kwe-concentration yeziyobisi, ebonisa ukuba i-protein ye-BAX ibonakaliswe ngokuhambelanayo.
Ixesha lokuposa: May-11-2023