[Abstract] Injongo Ukumisela i-N-terminal ye-15 intsalela ye-amino acid yamasuntswana amabini afanayo e-Agacutin.Amasuntswana amabini emolekyuli ye-enzyme kufuneka ahlulwe ukuze afumane ikhonkco lepeptide enye kwaye alandelelaniswe.Iindlela I-Agacutin ehlanjululwe kakhulu ifunyenwe ngendlela ye-biochemical.Iisampulu ze-subunit enye yokuchongwa kunye nokulandelelana zilungiswe ngu-SDS-PAGE ukuhlukana kwe-electrophoresis, i-subunit adhesive strip recovery kunye ne-PVDF inwebu ye-electrotransfer iteknoloji.Le yokugqibela inqume iintsalela ze-amino acid ezili-15 kwi-N-terminal ye-subunit nge-Edman degradation method.Iziphumo Ulandelelwano lweyunithi enkulu (16 kDa) yayiyi-DCSSGWSSYEEHQYY, kunye neyeyunithi encinci (15 kDa) yaba DSGWSSYEGHEYYV.Isiphelo Iziphumo zolandelelwano zibonise ukuba i-Agacutin yayiyi-thrombin entsha ye-snake venom njenge-enzyme ngokukhangela i-database ye-NCBI.
Ixesha lokuposa: Dec-22-2022