I-Snake venom thrombin njenge-enzyme (TLE) yi-serine protease yosapho lwe-trypsin, enolandelelwano olulondolozwe ngakumbi kunye ne-trypsin.Inomsebenzi we-arginine esterase, inokusebenza ngokuthe ngqo kwi-fibrinogen, i-catalyze i-cleavage ye-Arg2Gly peptide bond kwindawo ethile ye-molecule ye-fibrinogen, i-fibrinopeptide ikhulula i-fibrinopeptide A (FPA) okanye i-B (FPB. I-thrombins embalwa ye-alkaline yahlulwe yikholomu yokutshintshiselana kwe-cation, idibene Ukuhluzwa kwejeli kunye nechromatography ehambelanayo.I-chromatography ehambelanayo isetyenziswa kakhulu ngenxa yobuchule bayo bokuhlukana okuphezulu kunye nokubuyiselwa kwesampulu ephezulu.Kusetyenziswa iinkqubo ezahlukeneyo zokucoca, kwaye iimveliso ezifunyenweyo nazo zahlukile.. UGuo Chunteng et al. [6, 7] bafumana i-thrombin efana namacandelo I-P3 kunye ne-P4 ukusuka kwityhefu ye-Agkistrodon acutus e-Fujian ngokusebenzisa iinkqubo ezahlukeneyo zokucoca Uphononongo lubonisa ukuba zombini zinomsebenzi we-thrombin, kodwa i-EDTA ithintela ngokuyinxenye umsebenzi we-thrombin wecandelo le-P4 kwaye ivimbela ngokupheleleyo umsebenzi we-coagulase wecandelo le-P3. umsebenzi wokuvula into
Ixesha lokuposa: Dec-23-2022