I-antibody ye-IgY ngokuchasene ne-motif eqhelekileyo ye-metalloproteinases evela kwi-Agkistrodon acutus venom yalungiswa kwaye indima yayo kumsebenzi wetyhefu yenyoka yafundwa [Iindlela] Ngokuhlalutya ulandelelwano oluqhelekileyo lwee-metalloproteinases ezahlukeneyo ezivela kwi-Agkistrodon acutus venom, siyile kwaye senza i-antigenic peptides PT1 egcinwe kakhulu kunye ne-PT1. , ezihambelana ngokusondeleyo nemisebenzi yazo ye-enzyme;Iinkukhu ziye zagonywa kunye ne-conjugated complexes i-KLH-PT1 kunye ne-KLH-PT2 ukufumana i-antibodies ye-IgY.I-titer ye-IgY kunye neempawu ze-cross reaction kunye ne-venom ye-Agkistrodon acutus kunye ne-Agkistrodon brevius zafundwa kwangaphambili ngu-ELISA kunye ne-Western blot;Okokugqibela, umsebenzi wokungathathi hlangothi we-IgY wavavanywa ngovavanyo lwe-anti subcutaneous hemorrhage kwiimpuku [Iziphumo] i-ELISA kunye ne-Western blot yabonisa ukuba i-anti KLH-PT1 kunye ne-anti Agkistrodon acutus venom IgY inokunqumla kunye ne-Agkistrodon acutus venom kunye ne-Agkistrodon brevius venom, kunye ne-latter venom. yomelele kakhulu kuneyangaphambili;I-anti KLH-PT2 IgY yayingenayo impendulo ebalulekileyo enqamlezileyo kunye ne-Agkistrodon acutus kunye ne-Agkistrodon brevius venoms in vitro, kodwa yabonisa ubutyhefu obunamandla kuvavanyo lokuchasa ukopha kwi-vivo, kwaye yayiphezulu kakhulu kunezo zimbini zangaphambili [Isiphelo] I-antibody ye-IgY enako. Ukusabela komnqamlezo ngegazi elahlukileyo leetyhefu zenyoka ezijikelezayo zalungiswa ngokuyila i-antigen peptide ekwabelwana ngayo yi-metalloproteinases, eyabeka isiseko solungiselelo oluthile, olusebenzayo, lwetyhefu ephantsi kunye ne-anti-spectrum ebanzi ye-anti-hemorrhagic ye-snake venom antibody.
Ixesha lokuposa: Dec-26-2022