[Abstract] [Injongo] Ukulungiselela i-antibody ye-IgY ngokuchasene ne-motif eqhelekileyo ye-metalloproteinases evela kwi-Agkistrodon acutus venom, kunye nokufunda isiphumo sayo kumsebenzi wetyhefu yenyoka [IiNdlela] Ngokuhlalutya ulandelelwano oluqhelekileyo lwee-metalloproteinases ezahlukeneyo ezivela kwi-Agkistrodon acutus venom, siyile. kunye nokudibanisa i-antigen peptides egcinwe kakhulu ye-PT1 kunye ne-PT2, ehambelana ngokusondeleyo nomsebenzi we-enzyme;Iinkukhu ziye zagonywa nge-conjugated compounds KLH-PT1 kunye ne-KLH-PT2 ukufumana i-antibodies ye-IgY.I-titer ye-IgY kunye neempawu ze-cross-reaction kunye ne-Agkistrodon acutus kunye ne-Agkistrodon acutus venom zafundwa ngu-ELISA kunye ne-Western blot;Ekugqibeleni, umsebenzi wokungathathi hlangothi we-IgY wavavanywa ngovavanyo lwe-anti-subcutaneous hemorrhage kwiigundane [Iziphumo] Iziphumo ze-ELISA kunye ne-Western blot zibonise ukuba i-anti-KLH-PT1 kunye ne-anti-Agkistrodon acutus IgY inokuwela i-Agkistrodon acutus kunye ne-Agkistrodon acutus venom. , yaye le yokugqibela yomelele ngokuphawulekayo kuneyokuqala;I-Anti-KLH-PT2 IgY ayinayo intshukumo ecacileyo yomnqamlezo kunye ne-Agkistrodon acutus venom kunye ne-Agkistrodon acutus venom in vitro, kodwa inetyhefu eyomeleleyo kuvavanyo lokuchasa ukopha kwi-vivo, kwaye ingaphezulu kakhulu kunezo zimbini zangaphambili [Isiphelo] I-IgY. I-antibody enokuthi inqamleze isabele ngeetyhefu zenyoka ezijikeleza igazi yalungiswa ngokuyila ulandelelwano oluqhelekileyo lwe-antigen peptide ye-metalloproteinase, eyabeka isiseko solungiselelo oluthile, olusebenzayo, lwetyhefu ephantsi kunye ne-anti-spectrum ebanzi yokulwa ne-anti-hemorrhagic ye-antibody yetyhefu yenyoka.
Ixesha lokuposa: Jan-12-2023