[Abstract] Haemocoagulase Acutus (Halase), i-thermostatic entsha ekuphuhlisweni kwe-halase, yi-thrombobin esebenzayo ephezulu efana ne-enzyme (TLE) ephuma kwityhefu yenyoka ye-Agkistrodon acutus Ukufunda i-pharmacokinetics ye-Halase, indlela ye-radioisotope ene-125Ⅰ ebhalwe nge-radioisotope kunye ne-radiassay edityanisiweyo iprecip-itate yafakwa kwiimpuku.Iziphumo zibonise ukuba amaxabiso e-AUC adityaniswe kakuhle kunye needosi phantsi kweendlela zombini ezisetyenzisiweyo, kwaye i-coefficients yokulungelelanisa yayiyi-0.999 8 kunye ne-0.999 0 ngokulandelanayo;Ukuxinana kweziyobisi kwisibindi, udakada kunye nentliziyo kufikelele kwincopho yayo emva kwemizuzu emi-5 yolawulo ngelixa uninzi lwezinye ii-organs lufikelele kuyo ngemizuzu engama-30, emva koko lwehla kancinci;kwixesha ngalinye, ukuxinwa kweziyobisi esibindini kwakuphezulu kakhulu kunezinye izicubu;IHalase yakhutshwa ngokupheleleyo kwaye ikakhulu kumchamo.. I-Thrombin evela kwi-Agkistrodon acutus luhlobo lwe-thrombin ebekwe yodwa kwaye yacocwa kwityhefu ye-Agkistrodon acutus.Luhlobo olutsha lweziyobisi ze-hemostatic eziphuhliswe liqela lethu I-pharmacokinetics ye-thrombin esuka kwi-Agkistrodon acutus kwiigundane yafundwa yi-125 I-ileleble radioisotope method kunye ne-trichloroacetic acid precipitation edityaniswe nokufunyanwa kwe-radioactive.Iziphumo zibonise ukuba amaxabiso e-AUC alinganiswe ngeendlela ezimbini adityaniswe ngokufanelekileyo kunye nedosi, kwaye i-coefficients yokulungelelanisa yayiyi-0.9998 kunye ne-0.9990, ngokulandelanayo;Umxholo weziyobisi kwisibindi, i-spleen kunye nentliziyo yayiyeyona iphakamileyo ye-5 min emva kokulawulwa, ngelixa uninzi lwezinye izicubu zaziphezulu kwi-30 min emva kokulawulwa, kwaye emva koko ziyancipha ngokuthe ngcembe;Ngexesha ngalinye, umxholo weziyobisi kwizicubu zesibindi wawuphezulu kakhulu kunezinye izicubu;Ukongeza, i-thrombin excretion ye-Agkistrodon acutus igqityiwe, ngakumbi ngumchamo.
Ixesha lokuposa: Dec-08-2022