[Abstract] Injongo: Ukufunda umphumo we-Agkistrodon acutus venom suppressor component I (AAVC-I) kwi-proliferation inhibition kunye ne-apoptosis yomhlaza wemiphunga yabantu iiseli ze-A549.Iindlela: Amazinga okuvimbela i-AAVC-I kwiindawo ezahlukeneyo kwiiseli ze-A549 ze-24h kunye ne-48h zilinganiswa ngendlela ye-MTT;I-HE staining kunye ne-Hoechst 33258 fluorescence staining yasetyenziselwa ukujonga i-apoptosis evela kwi-morphology;Ukubonakaliswa kweprotheni ye-bax kwafunyanwa yi-immunohistochemistry.Iziphumo: I-MTT ibonise ukuba i-AAVC-I inokuthintela ukwanda kweeseli ze-A549 ngendlela exhomekeke kwixesha kunye ne-dose;Emva kokunyangwa kwe-AAVCI kwiiyure ze-24, i-pyknosis yenyukliya, i-nuclear hyperchromatic kunye nemizimba ye-apoptotic yabonwa phantsi kwe-microscope;I-Immunohistochemistry ibonise ukuba i-avareji ye-optical density inyuke ngokunyuka kwe-concentration yeziyobisi, ebonisa ukuba ukubonakaliswa kweprotein ye-bax yayilawulwa ngokuhambelanayo.Isiphelo: I-Antitumor component I ye-Agkistrodon acutus venom inokuthintela umhlaza wemiphunga womntu iiseli ze-A549 kwaye yenze i-apoptosis, enokuthi ihambelane nokulawulwa okuphezulu kwe-bax expression.
Ixesha lokuposa: Jan-18-2023