[Abstract] Injongo Ukwahlukanisa kunye nokucoca iprotein ye-anticoagulant esuka kwi-Agkistrodon acutus venom kumazantsi e-Anhui kunye nokujonga impembelelo yayo yokuthintela kwiiseli ze-vascular endothelial.Iindlela Iiseli ze-vascular endothelial ze-EC-304 zakhuliswa kwi-vitro.I-CCK-8 colorimetry yasetyenziselwa ukumisela i-cytotoxicity ye-anticoagulant protein fraction ye-Agkistrodon halys venom kwiiseli ze-EC-304 ezikhuliswe kwi-vitro.Isigaba sokuchasana kwemakroskopu sisetyenziselwe ukujonga utshintsho kwimopholoji yeeseli kunye nebhayomiji yeeseli ze-EC-304 ezinyangwa ngeqhezu leprotein elwa ne-anticoagulant ye-Agkistrodon halys venom.Iziphumo Iqhezu leprotein elichasayo le-Agkistrodon halys venom libe nothintelo olucacileyo lokukhula kwiiseli ze-EC-304 (P μ Iqhezu leproteni elichasayo le-Agkistrodon halys venom (g/mL) libe nefuthe elinamandla lokuthintela kwiiseli ze-EC-304, kunye nezinga lothintelo lonyukile. ngokunyuka kwethamo, iiseli ze-EC-304 zinyangwa nge-anticoagulant protein esuka kwi-Agkistrodon halys venom ibonise utshintsho lwe-morphological.Ngaphantsi kwe-microscope, iiseli zazingqukuva, inani leeseli ezinqunyanyisiweyo landa, inani lezigaba zokwahlulwa kweeseli lehla, kwaye ukuncipha kwenani leeseli ezinqunyanyisiweyo kukhula, Inani lamaqhekeza eeseli kwinkcubeko eliphakathi linyuke kakhulu.Isiphelo Iprotein ye-anticoagulant ye-Agkistrodon acutus venom evela kumazantsi ePhondo lase-Anhui inefuthe elinamandla lokuthintela kwiiseli ze-EC-304, kwaye i-apoptosis yeeseli ze-EC-304 inokuba yenye yeendlela zayo.
Ixesha lokuposa: Dec-29-2022