Isiphumo sokuNxinzelela iTumor icandelo I le-Agkistrodon acutus Venom kuMsebenzi wokuFudukela weVascular Endothelial Cells

[Abstract] Injongo: Ukuqwalasela isiphumo secandelo le-anti-tumor I ye-Agkistrodon acutus venom (AAVC-1) kumsebenzi wokufuduka we-umbilical vein endothelial cells (HUVECs), kunye nokuphonononga indlela enokwenzeka ye-AAVC - Ⅰ inhibiting. i-angiogenesis.Iindlela: I-HUVECs zaye zakhuliswa kwi-vitro kwaye zaphathwa nge-AAVC - Ⅰ (0, 20, 40, 80 μ G / ml) iiseli eziphathwayo zifakwe kwi-24 h.Uvavanyo lomkrwelo kunye novavanyo lwegumbi lekhemotactic zisetyenziselwe ukujonga ifuthe le-AAVC – Ⅰ kumsebenzi wokufuduka kweeseli ze-endothelial;I-RT-PCR kunye ne-Western blot zazisetyenziselwa ukufumanisa utshintsho lwe-mRNA kunye namanqanaba eprotheni ye-P-selectin kunye ne-intercellular adhesion factor (ICAM-1) ngaphambi nangemva kokunyanga kweziyobisi.Iziphumo: Xa kuthelekiswa ne-HU VECs kwiqela eliqhelekileyo, ukukwazi ukufuduka kweeseli kwi-AAVC - Ⅰ amaqela okuxininisa anciphile ukuya kuma-degrees ahlukeneyo, kwaye ukubonakaliswa kwe-P-selectin kunye ne-ICAM-1 mRNA yehla kakhulu.Isiphelo: I-AAVC - Ⅰ inokuthintela umsebenzi wokufuduka kweeseli ze-endothelial ngokunciphisa i-mRNA kunye namanqanaba eprotheni ye-P-selectin kunye ne-ICAM-1