Ukuqwalasela umphumo we-anti-tumor fraction I ye-Agkistrodon acutus venom (AAVC-1) kumsebenzi wokufuduka weeseli ze-umbilical vein endothelial cells (HUVECs), kunye nokuhlola indlela enokwenzeka ngayo ye-AAVC - Ⅰ inhibiting angiogenesis.Iindlela: I-HUVECs zaye zakhuliswa kwi-vitro nge-AAVC - Ⅰ (0, 20, 40, 80 μ G / ml) iiseli eziphathwayo zifakwe kwi-24 h.Uvavanyo lomkrwelo kunye novavanyo lwegumbi lekhemotactic zisetyenziselwe ukujonga ifuthe le-AAVC – Ⅰ kumsebenzi wokufuduka kweeseli ze-endothelial;I-RT-PCR kunye ne-Western blot zazisetyenziselwa ukufumanisa utshintsho lwe-mRNA kunye namanqanaba eprotheni ye-P-selectin kunye ne-intercellular adhesion factor (ICAM-1) ngaphambi nangemva kokunyanga kweziyobisi.Iziphumo: Xa kuthelekiswa ne-HU VECs kwiqela eliqhelekileyo, ukukwazi ukufuduka kweeseli kwi-AAVC - Ⅰ amaqela okuxininisa anciphile ukuya kuma-degrees ahlukeneyo, kwaye ukubonakaliswa kwe-P-selectin kunye ne-ICAM-1 mRNA yehla kakhulu.Isiphelo: I-AAVC - Ⅰ inokunciphisa ukubonakaliswa kwe-P-selectin kunye ne-ICAM-1 mRNA kunye
Ixesha lokuposa: Dec-21-2022