Injongo Ukuphanda isiphumo kunye nesigaba secandelo le-Agkistrodon acutus venom ACTX-8 ekunamatheleni kunye nohlaselo lomhlaza womlomo wesibeleko iseli yeHela.
Cima inkqubo.Iindlela zeFibronectin zagqunywa kwipleyiti yequla le-96.Iiseli zeHela zidlule kwi-0, 2.5, 5, 10, 20, 40 g/ml ye-ACTX-8
Emva kokonyango, ukunamathela kunye neFibronectin kwafunyanwa, kwaye inani leeseli ezinamathele lifunyenwe yi-MTY;Ukufunyanwa kokuhlaselwa kweeseli kunye nokufuduka ngendlela yeseli yeTransweU
Utshintsho kwizakhono;Ukubophelela okukhuphisanayo phakathi kwe-ACTX-8 kunye ne-Fibronectin kunye ne-Hela cell membrane yafunyanwa yi-cytometry yokuhamba.Iziphumo:
Emva konyango nge-ACTX-8, ukunamathela kweeseli ze-Hela kwi-Fibronectin kwehle, kwaye uvavanyo lweeseli ze-transwell lubonise ukuba iiseli ze-Hela zafuduka.
Amandla okufuduka kunye nokuhlasela nawo ancitshisiwe.Iziphumo ze-cytometry zokuhamba zibonisa ukuba i-ACTX-8 inokukhuphisana ukuze ibophe i-receptor ye-Fibronectin kwiseli.
Ixesha lokuposa: Feb-18-2023